Open Access

Margaret Buckingham, discoveries in skeletal and cardiac muscle development, elected to the National Academy of Science

Skeletal Muscle20122:12

DOI: 10.1186/2044-5040-2-12

Received: 17 May 2012

Accepted: 7 June 2012

Published: 7 June 2012

Abstract

Margaret Buckingham was presented as a newly elected member to the National Academy of Sciences on 28 April 2012. Over the course of her career, Dr Buckingham made many seminal contributions to the understanding of skeletal muscle and cardiac development. Her studies on cardiac progenitor populations has provided insight into understanding heart malformations, while her work on skeletal muscle progenitors has elucidated their embryonic origins and the transcriptional hierarchies controlling their developmental progression.

Keywords

National Academy of Sciences Cardiac development Skeletal muscle development

Commentary

Dr Margaret Buckingham, a much-respected investigator who has made many significant contributions to our understanding of skeletal muscle and cardiac development, was elected to the National Academy of Sciences in 2011 and presented on 28 April, 2012. Dr Buckingham is Professor in the Department of Developmental Biology at the Pasteur Institute in Paris. She has been awarded many prestigious distinctions including that of Officier de la Légion d’Honneur and Officier de l'Ordre National du Mérite, to name but two.

Dr Buckingham’s early studies involved the cloning and characterization of actin and myosin genes from cardiac and skeletal muscle [14]. She has made seminal contributions to our understanding of cardiac development. She identified the second heart field [5] and showed its important contribution to the poles of the heart. These cardiac progenitors are regulated by a distinct genetic network and in this context, Dr Buckingham has worked on the role of fibroblast growth factor (FGF) signaling in the formation of the outflow tract and pharyngeal arteries [6]. Her studies of cardiac development revealed that that two cell lineages contribute to the myocardium [7]. Lineage studies also demonstrated a clonal relationship between arterial pole myocardium and head muscles [8]. Her work on cardiac progenitor populations is of clinical importance in understanding heart malformations.

Dr Buckingham has also made major contributions to the molecular genetic analysis of skeletal muscle development. She was the first to analyze expression of the myogenic regulatory factors of the MyoD family during mouse embryogenesis [9] and the behavior of cells in the absence of Myf5 [10].

Her more recent work demonstrated that skeletal muscle growth depends on a somite-derived population of progenitor cells that express Pax3 and Pax7 [11]. She established that the Myf5 gene is activated by Pax3 through specific regulatory elements [12], and that Pax3 regulation of FGF signaling affects the balance between progenitor self-renewal and differentiation [13]. She showed genetically that before cells acquire myogenic potential, the equilibrium between Pax3 and Foxc2 expression in the somite regulates the choice between myogenic versus vascular cell fate [14]. After demonstrating the key role of satellite cells in adult muscle regeneration [15], she investigated satellite-cell quiescence, showing recently that microRNA31 targets Myf5 mRNA, and that both are sequestered in micro-ribonucleoprotein granules which breakdown on satellite cell activation [16]. She also identified microRNA-27 as a regulator of Pax3 production [17].

Dr Buckingham’s election to the academy recognizes her many significant contributions as a leading scholar in the molecular genetic study of striated muscle formation.

Declarations

Authors’ Affiliations

(1)
Ottawa Hospital Research Institute

References

  1. Minty AJ, Alonso S, Caravatti M, Buckingham ME: A fetal skeletal muscle actin mRNA in the mouse and its identity with cardiac actin mRNA. Cell 1982, 30: 185-192. 10.1016/0092-8674(82)90024-1View ArticlePubMedGoogle Scholar
  2. Robert B, Daubas P, Akimenko MA, Cohen A, Garner I, Guénet J-L, Buckingham M: A single locus in the mouse encodes both myosin light chains 1 and 3, a second locus corresponds to a related pseudogene. Cell 1984, 39: 129-140. 10.1016/0092-8674(84)90198-3View ArticlePubMedGoogle Scholar
  3. Robert B, Barton P, Minty A, Daubas P, Weydert A, Bonhomme F, Catalan J, Chazottes D, Guénet J-L, Buckingham M: Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross. Nature 1985, 314: 181-183. 10.1038/314181a0View ArticlePubMedGoogle Scholar
  4. Weydert A, Barton P, Harris AJ, Pinset C, Buckingham M: Developmental pattern of mouse skeletal myosin heavy chain gene transcriptsin vivoandin vitro. Cell 1987, 49: 121-129. 10.1016/0092-8674(87)90762-8View ArticlePubMedGoogle Scholar
  5. Kelly R, Brown N, Buckingham M: The arterial pole of the mouse heart forms fromFgf10expressing precursor cells in pharyngeal mesoderm.Dev Cell 2001, 1: 435-440. 10.1016/S1534-5807(01)00040-5View ArticlePubMedGoogle Scholar
  6. Watanabe Y, Miyagawa-Tomita S, Vincent SD, Kelly RG, Moon AM, Buckingham ME: Role of mesodermal FGF8 and FGF10 overlaps in the development of the arterial pole of the heart and pharyngeal arch arteries. Circ Res 2010, 106: 495-503. 10.1161/CIRCRESAHA.109.201665PubMed CentralView ArticlePubMedGoogle Scholar
  7. Meilhac SM, Esner M, Kelly RG, Nicolas J-F, Buckingham ME: The clonal origin of myocardial cells in different regions of the embryonic mouse heart. Dev Cell 2004, 6: 1-20. 10.1016/S1534-5807(03)00405-2View ArticleGoogle Scholar
  8. Lescroat F, Meilhac SM, Le Garrec JF, Nicolas JF, Kelly RG, Buckingham M: Clonal analysis reveals common lineage relationships between head muscles and second heart field derivatives in the mouse embryo. Development 2010, 137: 3269-3279. 10.1242/dev.050674View ArticleGoogle Scholar
  9. Sassoon D, Lyons G, Wright WE, Lin V, Lassar A, Weintraub H, Buckingham M: Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis. Nature 1989, 341:303-307. 10.1038/341303a0View ArticlePubMedGoogle Scholar
  10. Tajbakhsh S, Rocancourt D, Buckingham M: Muscle progenitor cells failing to respond to positional cues adopt non-myogenic fates in myf-5 null mice. Nature 1996, 384: 266-270. 10.1038/384266a0View ArticlePubMedGoogle Scholar
  11. Relaix F, Rocancourt D, Mansouri A, Buckingham MA: A Pax3/Pax7-dependent population of skeletal muscle progenitor cells. Nature 2005, 2005: 435,948-953.Google Scholar
  12. Bajard L, Relaix F, Lagha M, Rocancourt D, Daubas P, Buckingham ME: A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activatesMyf5in muscle progenitor cells in the limb. Genes Dev 2006, 20: 2450-2464. 10.1101/gad.382806PubMed CentralView ArticlePubMedGoogle Scholar
  13. Lagha M, Kormish JD, Rocancourt D, Manceau M, Epstein JA, Zaret KS, Relaix F, Buckingham ME: Pax3 regulation of FGF signaling as embryonic progenitor cells progress into the myogenic program. Genes Dev 2008, 22: 1828-1837. 10.1101/gad.477908PubMed CentralView ArticlePubMedGoogle Scholar
  14. Lagha M, Brunelli S, Messina G, Kume T, Relaix F, Buckingham ME: Pax3/7:Foxc2 reciprocal repression in the somite modulates multipotent stem cell fates. Dev Cell 2009, 17: 892-899. 10.1016/j.devcel.2009.10.021View ArticlePubMedGoogle Scholar
  15. Montarras D, Morgan J, Collins C, Relaix F, Zaffran S, Cumano A, Partridge T, Buckingham M: Direct isolation of satellite cells for skeletal muscle regeneration. Science 2005, 309: 2064-2067. 10.1126/science.1114758View ArticlePubMedGoogle Scholar
  16. Crist C, Montarras D, Buckingham M: Muscle satellite cells are primed for myogenesis, but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules. Cell Stem Cell 2012. In PressGoogle Scholar
  17. Crist CG, Rocancourt D, Montarras D, Buckingham M: Muscle stem cell behaviour is modified by microRNA-27 regulation of Pax3 expression. Proc Natl Acad Sci USA 2009, 106: 13383-13387. 10.1073/pnas.0900210106PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Rudnicki; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement