Table 1 Quantitive PCR primer sequences
From: A role for RNA post-transcriptional regulation in satellite cell activation
Gene | Primer type | Sequence |
|---|---|---|
GAPDH | Forward | 5’ - TGTGTCCGTCGTGGATCTGA - 3’ |
Reverse | 5’ - CCTGCTTCACCACCTTCTTGA - 3’ | |
18S | Forward | 5’ - GCCGCTAGAGGTGAAATTCTTG - 3’ |
Reverse | 5’ - CTTTCGCTCTGGTCCGTCTT - 3’ | |
Celf4 | Forward | 5’ CCTGCTCATCTACCATCTGCC - 3’ |
Reverse | 5’ - GCTCACGAAGCCAAAGCATTT - 3’ | |
Pabpn1 | Forward | 5’ - TTTCCTTGCCCTGTTTCCCATGTC - 3’ |
Reverse | 5’ - AGTGACTGAAGGGAGCACCTCAAA - 3’ | |
Ppargc1a | Forward | 5’ - TAGTTTGAGCCCTTGCTGGCTCTT - 3’ |
Reverse | 5’ - AGCTCAGTGAGGCTGATGTGTACT - 3’ | |
Mbnl1 | Forward | 5’ - AACTGGACAGAACCGGGAAGAACT - 3’ |
Reverse | 5’ - GCAAACTGCAACTTGTGACACGGA - 3’ | |
Matr3 | Forward | 5’ - ATTGTGGATAGGGCCAGTCATGGT - 3’ |
Reverse | 5’ - TTGCATTTGAGACAAGTGGCCTGG - 3’ | |
Sfrs3 | Forward | 5’ - TGTGGCACTGTGGGTGGAATGATA - 3’ |
Reverse | 5’ - CTGAAAGGACACTGGCATCTGAGT - 3’ | |
Zfp36 | Forward | 5’ - TCTCTGCCATCTACGAGAGCC - 3’ |
Reverse | 5’ - CCAGTCAGGCGAGAGGTGA - 3’ | |
Zfp36l1 | Forward | 5’ - GCTTTCGAGACCGCTCTTTCT - 3’ |
Reverse | 5’ - TTGTCCCCGTACTTACAGGCA - 3’ | |
Zfp36l2 | Forward | 5’ - AGCGGCTCCCAGATCAACT - 3’ |
Reverse | 5’ - CGAAAGCGAAGGCGTTGTTA - 3’ | |
Elavl1 | Forward | 5’ - TGTGAGTCACCAGCTGCCAAGTAT - 3’ |
Reverse | 5’ - GAGGTGGTTCAAACCAACCAACCA - 3’ | |
Cdk2 | Forward | 5’ - TCCTCTGAGAGCAGTGATGCA - 3’ |
Reverse | 5’ - TTCCCCCAATGACCTAACCAG - 3’ | |
E2F3 | Forward | 5’ - GGTCCTGGATCTGAACAAGGC - 3’ |
Reverse | 5’ - CCTTCCAGCACGTTGGTGAT - 3’ | |
U6 | Reverse | 5’ - AATTCGTGAAGCGTTCCATAT - 3’ |
miR-16 | Reverse | 5’ - TAGCAGCACGTAAATATTGGCG - 3’ |
miR-93 | Reverse | 5’ - CAAAGTGCTGTTCGTGCAGGTAG - 3’ |
miR-106b | Reverse | 5’ - TAAAGTGCTGACAGTGCAGAT - 3’ |
miR-107 | Reverse | 5’ - AGCAGCATTGTACAGGGCTATCA - 3’ |
miR-124 | Reverse | 5’ - TAAGGCACGCGGTGAATGCC - 3’ |
miR-200b | Reverse | 5’ - TAATACTGCCTGGTAATGATGA - 3’ |