Figure 2

Canine derived Pax7-positive cells persist in injected mouse muscle. (A) Cryosections from mouse muscle injected with canine muscle-derived mononuclear cells were immunostained with anti-Pax7 (green), anti-lamin A/C (red), and anti-laminin (blue). (B) The number of nuclei expressing canine lamin A/C and Pax7 per cross-section was counted, and the points on the graph represent the average of the averages ± SD, where the average was calculated from three cryosections per mouse, and the average of the averages was calculated from at least three mice per cell dose, and at least two separate cell isolations per cell dose. (C) Satellite cell-derived myoblasts were isolated and cultured from canine muscle, and mouse muscle injected with 400, 2000, or 10,000 canine muscle-derived mononuclear cells. The cells were fixed and immunostained with anti-lamin A/C (red), and anti-Pax7 and anti-myogenin (both green).