Figure 1

Progression of dystrophic histopathology in the third compartment of the extensor digitorum longus (EDL) muscle. (A) Hematoxylin and eosin stained frozen sections from 3-month-old wild-type and mdx^4cv muscle. Note the central nuclei in the mdx^4cv myofibers. Scale bar = 50 μm. (B) Proportion of central nuclei (+/- S.D.) in the third EDL compartment from 3 weeks to 20 weeks of age (n = 4-6). Note the apparent stabilization of dystrophic histopathology from 12 to 20 weeks of age. ***P <0.001 at 12 weeks compared to 4 weeks of age. At some time points, the standard deviation is too small to see. (C) The mass of the third compartment of the EDL at 20 weeks of age. P <0.001 compared to wild-type. (D) Box-whiskers plot of median +/- 75% of myofiber areas in wild-type and mdx mice at 20 weeks of age.