Figure 9
Quantitative RT-PCR of angiotensin-related genes shows dysregulation of the renin-angiotensin signaling system in DyW muscle. (A) Gene analysis of angiotensin (Agt) shows no difference in expression between Lama2DyW (DyW) and wild-type (WT) animals from postnatal weeks 1 through 4. (B) We found that angiotensin-converting enzyme (Ace) was significantly upregulated by week 3 (P < 0.005, by two-way analysis of variance (ANOVA) (n = 5)). (C) Angiotensin II type 1a receptor (Agtr1a) was also upregulated starting at week 3 in these mice (P < 0.001 by two-way ANOVA (n = 5). (D) We found that the expression of angiotensin II type 2 receptor (Agtr2) was increased several-fold over the WT mice starting at week 2 (P < 0.0001 by two-way ANOVA (n = 5)). Note that, though it does not entirely come across in the graph, there is a 25-fold increase in Agtr2 expression at week 4. Asterisks indicate statistical significance between age-matched DyW and WT animals, hash tags indicate significant changes within the DyW group and Deltas indicate significant changes within the WT group. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. These statistical representations also apply other symbols (Δ, #).