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Table 3 Transcripts modulated in A/J vs. WT myoblasts co-cultured with M2a macrophages

From: Upregulated IL-1β in dysferlin-deficient muscle attenuates regeneration by blunting the response to pro-inflammatory macrophages

Gene symbol

Description

WT-M2 vs. WT

AJ vs. WT

AJ-M2 vs. AJ

Myl2

Myosin, light polypeptide 2, regulatory, slow

2.31

n/a

3.07

Sdc4

Syndecan 4

2.04

−2.16

n/a

Nrap

Nebulin-related anchoring protein

1.98

n/a

2.29

Calr

Calreticulin

1.88

n/a

−1.87

Mylk2

Myosin light chain kinase 2

1.88

n/a

n/a

Ltbp1

Latent transforming growth factor beta binding protein 1

1.85

1.54

n/a

Ckm

Creatine kinase, muscle

1.83

n/a

1.78

Mybpc2

Myosin binding protein C, fast type

1.64

2.47

1.94

Rhob

Ras homolog family member B

1.62

n/a

1.58

Myh7

Myosin, heavy chain 7 (type I)

1.61

−1.87

n/a

Il4

Interleukin 4

1.59

n/a

n/a

Ryr1

Ryanodine receptor 1

1.57

n/a

n/a

Myoz2

Myozenin 2

1.56

−1.69

1.81

Myh6

Myosin, heavy chain 7 (cardiac)

1.52

n/a

2.00

Myom1

Myomesin 1

1.52

n/a

1.54

Id2

Inhibitor of DNA binding 2

−1.56

1.77

−2.20

Il7

Interleukin 7

−1.57

1.70

n/a

Rab27b

RAB27B, member RAS oncogene family

−1.61

n/a

−1.67

Iigp2

Interferon inducible GTPase 2

−1.79

2.06

−2.83

Psmb8

Proteosome subunit, beta type 8

−1.84

1.78

−1.86

Ifi47

Interferon gamma inducible protein 47

−1.90

2.18

−2.00

C2

Complement 2

−1.95

2.64

−2.40

Casp1

Caspase 1

−2.18

1.98

−2.22

Plk1

Polo-like kinase 1

−2.29

−2.76

−1.68

Irf1

Interferon regulatory factor 1

−2.91

1.88

−2.43

Gbp2

Guanylate binding protein 2, interferon-inducible

−3.37

1.60

−2.33

Akt2

v-akt murine thymoma viral oncogene 2

n/a

n/a

1.51

Gsk3B

Glycogen synthase kinase 3

n/a

n/a

−1.75

Itgb1

Integrin, beta 1 (fibronectin receptor beta)

n/a

n/a

−2.37

Jak1

Janus kinase 1

n/a

n/a

−1.87

Myoz1

Myozenin 1

n/a

n/a

1.70

Ripk1

Receptor (TNFRSF)-interacting serine-threonine kinase 1

n/a

n/a

−1.51

Vegfc

Vascular endothelial growth factor C

n/a

n/a

−1.50

Slc1a3

Solute carrier family 1 (glial high affinity glutamate transporter), member 3

n/a

5.59

−1.98

H2-T23

Histocompatibility 2, T region locus 23

n/a

4.41

−1.66

Il1b

Interleukin 1 beta

n/a

3.05

−2.47

Cxcl16

Chemokine (C-X-C motif) ligand 16

n/a

2.92

−1.74

Col5a2

Collagen, type V, alpha 2

n/a

2.66

−1.72

Crlf1

Cytokine receptor-like factor 1

n/a

2.64

−1.69

Ccl9

Chemokine (C-C motif) ligand 9

n/a

2.00

1.50

Angptl4

Angiopoietin-like 4

n/a

1.93

−1.52

Stat1

Signal transducer and activator of transcription 1

n/a

1.77

−1.61

Tnfrsf1b

Tumor necrosis factor receptor superfamily, member 1b

n/a

1.63

−1.51

Bmpr2

Bone morphogenetic protein receptor 2

n/a

1.58

−1.61

Stam2

Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2

n/a

1.57

−1.62

Gvin1

GTPase, very large interferon inducible 1

n/a

1.53

−1.75

Tnfsf9

Tumor necrosis factor (ligand) superfamily, member 9

n/a

1.51

−1.57

Prkaca

Protein kinase, cAMP-dependent, catalytic, alpha

n/a

−1.58

1.51

Prosapip1

ProSAPiP1 protein

n/a

−1.90

1.67

Myf6

Myogenic factor 6

n/a

−3.54

2.62

Musk

Muscle, skeletal, receptor tyrosine kinase

n/a

−4.73

−1.53

Cdkn1c

Cyclin-dependent kinase inhibitor 1C (p57, Kip2)

n/a

−18.11

5.52

  1. Fold-change values of Illumina Gene array transcript subset which showed significant modulation by WT-M2a co-culture vs. WT alone compared to their fold-change values in A/J vs. WT and A/J-M2a co-culture vs. A/J alone. Only transcripts with fold-change values >1.5 and P values <0.01 were considered for analysis. ANOVA was applied to verify significance