Fig. 8
From: Regions of ryanodine receptors that influence activation by the dihydropyridine receptor β1a subunit
Effect of β1a subunit on RyR1 in lipid bilayers is abolished for the K-Q mutation. a–c (left) Average relative P o (log10 rel P o ; a), mean open time (log10 To; b) or mean closed time (log10 rel T c ; c) were calculated in the same ways as described for averaged relative P o in Fig. 2a, left. a–c (right) The average of the single channel parameter values shown to the right of the corresponding relative values. a–c Single-channel parameters were calculated from ~180 s of channel activity (at +40 and −40 mV). Data are shown without β1a (0 nM β1a) (black bar), 10 nM β1a subunit (dark grey bar), and 100 nM β1a subunit (light grey bar), where applicable. Error bars indicate ± −SEM. n = 5–14 experiments/bar. *p < 0.05 vs control determined by paired (left) or un-paired (right) Student’s t-test, # p < 0.05 vs WT RyR2 determined by ANOVA