Fig. 4

Novel genotyping PCR of Dysf ^tm1Kcam. a Schematic drawing of the targeted dysferlin locus in wild type and Dysf ^tm1Kcam mice, with binding sites of primers used for genotyping shown in pink. The genotyping primers previously described by Han et al. [12] are depicted in blue (#2290, #2581, #4768). b Results of a genotyping PCR using DNA from toe biopsies of homozygous mutant (Dysf ^{tm1Kcam/tm1Kcam}), heterozygous mutant (Dysf ^tm1Kcam/+), and wild type mice, as well as a no template control (NTC)