Fig. 3

TSCmKO but not RAmKO mice show changes in non-targeted metabolic organs. a Immunoblots of TA muscle from 10-week-old TSCmKO, RAmKO, and control (Ctrl) mice are shown for the indicated phospho (P)- and total proteins (n = 4). Protein expression is normalized to α-actinin. Quantification of phosphorylation is shown relative to the total amount of each protein except for P-S6. b Glycogen amount is increased in the gastrocnemius muscle of 12-week-old RAmKO mice (n = 5) compared to TSCmKO (n = 7) and control (Ctrl) mice (n = 13). c Liver lipid content is decreased in 12-week-old TSCmKO as shown by Oil Red O staining, while it is unchanged in 10-week-old RAmKO mice (n = 3). Scale bar: 100 μm. d The concentration of non-sterified fatty acids in the plasma is decreased in 10-week-old TSCmKO mice (n = 4) compared to RAmKO (n = 6) and control (Ctrl) mice (n = 8). e Immunoblots of the liver from 10-week-old TSCmKO, RAmKO, and control (Ctrl) mice are shown for the indicated phospho (P)- and total proteins (n = 4). Protein expression is normalized to β-actin. Quantification of the phospho-protein is shown relative to the amount of each protein. f Glycogen amount is decreased in the liver from 12-week-old TSCmKO mice (n = 4) compared to RAmKO (n = 3) and control (Ctrl) mice (n = 6). Data presented are all from male mice of the indicated genotypes. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001