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Fig. 2 | Skeletal Muscle

Fig. 2

From: The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle

Fig. 2

Validation of Stac3 knockout. a Analysis of Stac3 mRNA expression. Stac3 mRNA in the hindlimb and forelimb muscles of Stac3 fl/fl, Stac3 +/fl Tg Cre, and Stac3 fl/fl Tg Cre mice at 8 weeks of age, i.e., 4 weeks after tamoxifen injection, was quantified by RT-qPCR. Bars not sharing the same letter labels are different (P < 0.05, n = 4 mice). b Images from western blotting analyses of STAC3 protein in hindlimb (HL) and forelimb (FL) skeletal muscles of 8-week-old Stac3 fl/fl, Stac3 +/fl Tg Cre, and Stac3 fl/fl Tg Cre mice. β-tubulin (TUBB) protein was used as a loading control. c Quantification of STAC3 protein band intensity. The expression level of STAC3 protein is normalized to that of β-tubulin in the same sample. Bars not sharing the same letter labels are different (P < 0.05, n = 3 mice)

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Skeletal Muscle

ISSN: 2044-5040

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