Fig. 2

Histological analysis of the diaphragm muscle isolated from mdx treated with rGDF11 or vehicle. a Representative images of the diaphragm muscle using H&E staining from mice treated with rGDF11 or vehicle. The treatment with GDF11 did not alter the percentage of centrally nucleated fibers. b Representative images of the diaphragm muscle using Masson’s trichrome staining from mice treated with rGDF11 or vehicle. No differences were observed in terms of the percentage of fibrotic tissue upon rGDF11 treatment as determined by the quantification of the blue staining area (b). Fiji ImageJ software was used to quantify the percentage of collagen area. Scale bar = 100 μm