Fig. 5
From: Characterization of a Dmd EGFP reporter mouse as a tool to investigate dystrophin expression
Dystrophin-EGFP expression in the brain. Immunofluorescent staining of brain sections with the Dys2 antibody (red) shows colocalization of the natural EGFP signal (green) with the dystrophin signal in the cerebellum of Dmd EGFP mice. Partial colocalization of the EGFP signal with the anti-CD31 (red) signal confirmed the presence of dystrophin expression also in the blood vessels of the brain (second column). Open arrowheads show the EGFP signal surrounding the signal from the CD31 marker. Immunofluorescent staining with anti-glial fibrillary acidic protein (GFAP, red) shows colocalization with the EGFP signal at the glial endfeet in the cerebellum (third column). A higher magnification of the anti-GFAP signal is shown in the fourth column. EGFP expression is confined to the glial endfeet (asterisk), whereas the green fluorescent tag is not expressed in the glial cell body (closed arrowheads)