Fig. 2

The impact of increasing ACh was determined by recording miniature endplate potentials (MEPPs) in the diaphragm of young adult control and VAChT^Hyp mice. The frequency of MEPPs is unchanged in VAChT^Hyp mice (a), with error bar representing the standard error. However, the average MEPP amplitude is higher in VAChT^Hyp (n = 2297 events) compared to that in control mice (n = 2444 events) (b–d). The amplitude of MEPPs was on average significantly higher in VAChT^Hyp mice using a Kolmogorov-Smirnov test (d; P value <0.001). Results are from 50 muscle fibers from five mice per genotype. Error bar = Standard Error. Four-month-old male mice on a C57BL/6 background were used for these experiments