Fig. 5From: VAChT overexpression increases acetylcholine at the synaptic cleft and accelerates aging of neuromuscular junctionsAccelerated aging of NMJs in aging VAChTHyp mice. α-motor nerve endings (green in a and b) were visualized using an antibody against synaptotagmin-2 (Syn-2; green). There were more fragmented and denervated NMJs found in 19-month-old VAChTHyp (b–d) compared to control (a, c–d) mice of the same age and sex. Four mice per genotype were examined and at least 50 NMJs per animal analyzed. Scale bar = 20 μm. Error bar = Standard Error. *P value <0.05, **P value <0.01Back to article page