Fig. 1

Macrophages and tissue necrosis prior to and in early days after cardiotoxin injury in mTG mice. a FACS analysis of uninjured muscles of WT and mTG animals, showing the proportion of pan-macrophages (F4/80⁺ cells), M1 macrophages (F4/80⁺CD80⁺CD163^- cells), and M2 macrophages (F4/80⁺CD80⁻CD163⁺ cells); n = 5 per group; b proportion of pan-macrophages and M1 and M2 subpopulations in TA muscles of WT and mTG animals 1 and 3 days after CTX injection using FACS analysis; n = 5–7 per group; c relative gene expression levels of M1 and M2 macrophage markers in baseline condition (n = 5–6 per group) as well as 1 day (n = 3–4 per group) and 3 days (n = 5–7 per group) post-CTX-injection; d representative immunohistochemistry (IHC) images (CD68: green; dapi/nuclei: blue; laminin: gray) and corresponding H&E sections showing accumulation of macrophages (green in IHC images) in actively regenerating areas (dark purple in H&E staining); e measurement of area occupied by M1 macrophages from IHC images; n = 7–8 per group; f measurement of necrotic area from H&E sections normalized to total section area; n = 7–8 per group (t test); values are plotted as individual values or average ± SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001