Fig. 6
From: A novel method for the quantification of fatty infiltration in skeletal muscle
Quantification of lipid volume by optical density (OD) measurement of ORO content in extracted lipid. a Regression of ORO extraction vs. lipid volume measurements via fluorescent confocal microscopic analysis (data from Fig. 5). ORO OD readings are significantly correlated (p < 0.0001) with lipid volume measurements made prior to ORO staining. Inset shows a magnified view of the low lipid volume data points. b ORO OD readings are sensitive enough to detect a significant difference between strains. ***p < 0.0005