Table 1 RT-PCR primers for SSPN, μSPN, and nSPN amplification
Primer name | Sequence (5′ ➔ 3′) | NCBI accession no. | Usage | Location within SSPN | Cross-reactivity | Species |
|---|---|---|---|---|---|---|
SSPNATGF | ATGGGCAAGAACGAGCAG | AF016028 | Forward primer, 1st and 2nd PCR | Exon 1 | SSPN, μSPN, and nSPN | Human |
Ex3vReverse | TCCATTCCAAATGGCTTGAT | EU433933 | Reverse primer, 1st PCR | 3′ UTR (26 nt downstream of stop codon) | μSPN, nSPN | Human |
Ex1/4R | TCAGAAGTCATGACAATGGATAA | EU433933 | Reverse primer, 2nd PCR; probe, Northern blot | Exon 1 and exon 4 boundary | μSPN, nSPN | Human |
SSPN118F | CTGCTAGTCAGGGACACTC | AF016028 | Forward primer, RT-PCR | Exon 1 | SSPN, μSPN, and nSPN | Human |
Ex4R | TTGATTCTGAGAACATTCTCTTTC | EU433933 | Reverse primer, RT-PCR | 3′ UTR (7 nt from stop codon) | μSPN, nSPN | Human |