Fig. 2
Expression of C5a and C5aR1 in tibialis anterior (TA) muscle of wild-type (WT) and hSOD1G93A (SOD1) mice at three different stages of disease progression. a shows the protein expression of C5a in the TA muscle of WT (white bars) and SOD1 (black bars) mice at onset (OS; postnatal day 70 (P70)), mid-symptomatic (MS; postnatal day 130 (P130)) and end-stage of disease (ES; postnatal day 175 (P175). b shows mRNA expression of C5aR1 in the TA muscle of SOD1 mice relative to age matched WT mice at three different ages. Top panel in c shows a representative western blot of C5aR1 with GAPDH in the TA muscle of SOD1 mice relative to age matched WT mice at three different ages and soleus (SOL) muscle of SOD1 mice relative to age matched WT mice at end stage of disease. Bottom panel in c shows protein expression of C5aR1 determined by semi-quantitative densitometry in the TA muscle of SOD1 mice relative to age matched WT mice at three different ages and SOL muscle of SOD1 mice relative to age matched WT mice at end stage of disease. Data in a–c are expressed as means ± SEM (n = 6 mice/group; * p < 0.05, ** p < 0.01, *** p < 0.001, Student t test at each stage)