Fig. 3
C5aR1 is not localised at the neuromuscular junction or Schwann cells in the tibialis anterior (TA) muscle of wild-type (WT) and hSOD1G93A (SOD1) transgenic mice. Panels a to p shows double immuno-labelling of C5aR1 (red) with cellular markers (green) for neuromuscular junction (\( \boldsymbol{\upalpha} \)BTX; a to c for WT (detailed in d); e to g for SOD1 mice (detailed in h)) and Schwann cells (S100β; i to k for WT (detailed in l); m to o for SOD1 mice (detailed in p)) in the TA muscle of WT and SOD1 mice at end-stage of disease. C5aR1 is not localised to neuromuscular junction (yellow arrows; d and h) and Schwann cells (yellow arrows; l and p) as indicated by lack of co-localisation with \( \boldsymbol{\upalpha} \)BTX and S100β. Scale bar for all panels = 20 μm