Fig. 5

Reduction of CD11b⁺/CD68⁺ macrophages in tibialis anterior (TA) muscle of hSOD1^G93A mice lacking C5aR1 (SOD1 × C5aR1^-/-) when compared to hSOD1^G93A (SOD1) mice. TA muscle from wild-type (WT), SOD1 and SOD1 × C5aR1^-/- mice were stained for CD11b and CD68 for macrophages. a and b Representative images of CD11b and CD68 in WT, SOD1 and SOD1 x C5aR1^-/- mice at onset, mid-symptomatic and end-stage of disease. Scale bar = 20 μm. c More CD11b⁺ macrophages were present in SOD1 mice at onset (OS; P70), mid-symptomatic (MS; P130) and end-stage of disease (ES; P175) when compared to WT mice, while a reduction in CD11b⁺ macrophages were present in SOD1 × C5aR1^-/- mice at MS and ES when compared with SOD1 mice (n = 3; * p < 0.05, *** p < 0.001, one-way ANOVA with Tukey’s post hoc test at each stage of disease). d CD68⁺ macrophages were also increased in SOD1 mice compared to WT mice at MS and ES with a decrease in SOD1 × C5aR1^-/- at these stages (n = 3, * p < 0.05, one-way ANOVA with Tukey’s post hoc test at each stage of disease). Data in c and d are expressed as means ± SEM