Fig. 6
Ablation of C5a-C5aR1 signalling in hSOD1G93A (SOD1) mice reduces neuromuscular (NMJ) denervation and improves hind-limb grip strength. Panels a to c shows double immuno-labelling of neuromuscular junction (\( \boldsymbol{\upalpha} \)BTX; red) with pre-synaptic marker (SNP; green) for WT (a), SOD1 (b) and SOD1 x C5aR1-/- (c) mice at mid-symptomatic stage of disease. d SOD1 x C5aR1-/- mice showed reduction in NMJ denervation when compared to SOD1 mice at mid-symptomatic stage of disease (n = 3, * p < 0.05 and ** p < 0.01, one-way ANOVA with Tukey’s post hoc test). e This was further supported by hind-limb grip strength, where SOD1 x C5aR1-/- mice showed improvement in motor deficits when compared to SOD1 mice at mid-symptomatic stage of disease (n = 10, * p < 0.05 and *** p < 0.001, one-way ANOVA with Tukey’s post hoc test). Data in d and e are expressed as means ± SEM. Scale bar = 20 μm