Fig. 6

Loss of CD82 expression in dystrophic muscle leads to increased mTOR phosphorylation at Ser 2448. Quadriceps muscle extracts from 12-week-old male mdx^5cv and CD82^−/−:mdx^5cv mice were analyzed by Western blot and probed for multiple signaling proteins known to control cell size and quantified. a Quantification of PTEN and pAKT (Ser 473) protein levels did not show significant differences between mdx^5cv and CD82^−/−:mdx^5cv muscle extracts. b Immunoblots for mTOR (pSer 2448) and mTOR (total) show an increase of mTOR-phosphoSer2448 in CD82^−/−:mdx^5cv compared to mdx^5cv muscles extracts (*p = 0.02). c Analyses of p-p70S6K and p4EBP1, two known targets of mTOR signaling normalized to their total non-phosphorylated forms show no significant differences between the genotypes. N = 5/genotype