Fig. 8

Recapitulation of TH pathway effects with MCT10 knock-down. A Gene expression in TA muscles electroporated with siRNA against MCT10 or a non-silencing RNA duplex. Expression levels of MCT10, targeted by the siRNA employed, and those of the related gene MCT8 and Six1 are shown. mRNA levels are normalized to the geometric mean of 18S and Actnb. Data were obtained from 4 biological replicates (animals). Error bars indicate the mean ± SEM of the replicates. P values in red indicate statistically significant changes in levels of expression as determined by one-tailed paired Student’s T tests relative to siNS (p value < 0.05). B mRNA expression level of a panel of four TH pathway target genes in the control and MCT10 knockdown samples from panel A. Two-way ANOVA (testing mRNA levels as a function of which gene was tested and which siRNA was received) indicates significant reduction of the TH signaling gene panel with MCT10 knockdown (p value = 0.005). C MCT10 knock-down leads to a decrease in TH-dependent gene transcription. Relative TRE-dependent luciferase activity in TA muscle electroporated with siRNA targeting MCT10. N = 5 biological replicates. The decrease in reporter gene expression is significant as determined by paired one-tailed T test, compared to non-silencing control (siNS)