Fig. 2
From: The Myotube Analyzer: how to assess myogenic features in muscle stem cells
Nucleus indication. The upper panel shows both the original input image, as well as the “adjusted” image in the blue channel (nuclear staining using Hoechst). The lower panel shows editing controls, statistics panel and output of the “Nuclei indication” function. Nuclei are indicated with yellow asterisks and with red asterisks if they are positive for the marker in the green channel (due to the MyoD staining in this case). The right image does not show nuclei outside of the mask, meaning outside of the myotubes (based on MyHC, red channel). Centroids can be added or removed with the editing functions, using either the left or the right image as a guide