Fig. 9

Partial co-localization of DUX4c with RNA-binding proteins IMP1, SFPQ, or FUS in FSHD muscle cells. FSHD primary myoblasts were fixed in PAF and immunofluorescence to detect DUX4c was performed as in Fig. 3 with the additional primary serum against either IMP1, SFPQ, or FUS and the appropriate secondary antibodies coupled to different Alexa Fluor with the indicated colors. Staining was observed by epifluorescence microscopy. The nuclei were labeled with DAPI. Partial cytoplasmic co-localization of DUX4c and the mRNP granule markers IMP1 and SFPQ (A, arrows) or IMP1 and FUS (B, circle). The arrows point to regions apparently at the tip of elongating cells (stars). Box shows an intense DUX4c staining without RBP co-detection. N = 3 biological replicates