Fig. 3

Effect of critical illness and loss of FGF21 on muscle histology. a Tibialis anterior sections immunostained for laminin. b Tibialis anterior myofiber size distribution. c Illustration of tibialis anterior general histology after hematoxylin–eosin staining. d Semi-quantitative scoring of tibialis anterior histology. Data are shown as cumulative percentages of the respective group. Healthy control, Fgf21^+/+: n = 11 at 30 h, n = 20 at 5 days; Healthy control, Fgf21^−/−: n = 11 at 30 h, n = 23at 5 days; Critically ill, Fgf21^+/+: n = 15 at 30 h, n = 19 at 5 days; Critically ill, Fgf21^−/−: n = 15 at 30 h, n = 18 at 5 days. * p < 0.05, *** p < 0.001 between healthy control and critically ill mice