Fig. 2

Global poly(ADP-ribosylation) is increased in striated muscles of golden retriever muscular dystrophy. A Representative immunoblots showing total PARylated protein level expression with PAR antibody in cardiac and skeletal muscle from early and advanced age WT and GRMD dogs. Ponceau was shown as loading control. Bars indicate mean ± standard error of mean (relative to ponceau), and numbers above columns indicate P-values (n = 3 dogs for each condition). Differences were analyzed by unpaired t-test. B Representative immunoblots showing PARP-1 protein level expression in cardiac and skeletal muscle from early and advanced age WT and GRMD dogs. Ponceau was shown as loading control. Bars indicate mean ± standard error of mean (relative to ponceau), and numbers above columns indicate P-values (n = 3 dogs for each condition). Differences were analyzed by unpaired t-test