Fig. 3

Distinct transcriptomic profiles in ∆Ig3-MuSK TA and soleus. WT and ∆Ig3-MuSK soleus and TA muscles were subjected to RNA sequencing at a depth of ~ 50 million reads per sample (n = 9 muscles/genotype; one muscle/animal). Heat map of the top 1000 variable genes in WT and ∆Ig3-MuSK TA (A) and soleus (B). Each column represents one muscle sample. Principal component analysis of WT and ∆Ig3-MuSK TA (C) and soleus (D) shows clustering of samples with similar gene expression profiles (▲, WT; ●, ∆Ig3-MuSK; individual muscles). E and F Differentially expressed genes (DEGs) with 1.2 minimum fold change and adjusted p-value < 0.05 were determined using DEseq2 (see also Tables S1, S2). G and H Volcano plot of -log10 FDR versus gene log2 fold change (red dots up DEGs; blue dots down DEGs). I Venn diagram depicting the shared and unique upregulated and downregulated DEGs between ∆Ig3-MuSK TA and soleus