Fig. 7
From: The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signaling
Akt-mTOR signaling is reduced in ∆Ig3-MuSK soleus but not TA. A WT and ∆Ig3-MuSK soleus muscle protein extracts were isolated for Western blotting of pSmad1/5, total Smad1, p-4EBP1, and total 4EBP1. Total protein stain was used as loading control and for protein normalization. Levels of pSmad1/5 and p4EBP1 were determined as a ratio to total Smad1 and total 4EBP1, respectively. Note that p-4EBP1 levels were reduced in ∆Ig3-MuSK soleus compared to WT (C) but unchanged in TA (D). pSmad 1/5 levels in ∆Ig3-MuSK soleus and TA were comparable to WT (E and F). Data are means ± SEM from five biological replicates (*p < 0.05, unpaired two tailed Student’s t-test)