Fig. 7

VDR helps mediate the anti-atrophy effects of eldecalcitol in TNF-α treated myotubes. a–c Eldecalcitol increased the interaction of VDR with P65 and P52. C2C12 myotubes were stimulated with TNF-α (100 ng/ml) for 24 h in the presence or absence of eldecalcitol (10 nM). d–g Eldecalcitol-mediated anti-atrophy effects were partly blunted by VDR gene silencing in myotubes exposed to TNF-α. Values are presented as mean ± SEM, n ≥ 3. *P < 0.05; **P < 0.01; ***P < 0.001. Con, control; ELD, eldecalcitol; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SEM, standard error of mean, VDR, vitamin D receptor