Fig. 2

The SOMAmer detects the previously characterized N-terminal fragment of titin in DMD patient urine. A SOMAmer detects the N-terminal fragment (amino acids 1–194) of titin. B Western blot using anti-TTN mouse monoclonal antibody [clone: 7D3] against amino acids 1–110 shows the presence of the fragment in DMD (lanes 7–14) urine, as well its absence in age-matched healthy controls (lanes 1–6). C Human urine ELISA confirms increases in the titin fragment seen in the SOMAscan panel and Western blot. D Normalization of the ELISA results using creatinine, E specific gravity, and F cystatin C show significant increases in the urinary titin fragment in DMD urine. For individual plots of normalization values, see Supplementary Fig. 1