A knock down strategy for rapid, generic, and versatile modelling of muscular dystrophies in 3D-tissue-engineered-skeletal muscle

Table 1 Viral constructs used in this study

Lentiviral construct	Insert or shRNA target sequence	Experiment	Vector origin
H2B-GFP	H2B-GFP	Figures 1 and S1
Non-targeting shRNA	AACAAGATGAAGAGCACCAACT	Figures 2, 3, 4 and S2–S9	MISSION-shRNA library; SHC002
CAPN3-targeting shRNA #1	CCGCAACTTCCCAGATACTTT	Figure S2	MISSION-shRNA library; TRCN0000003493
CAPN3-targeting shRNA #2	CGGAGTGAAAGAGAAGACATT	Figure S2	MISSION-shRNA library; TRCN0000003494
CAPN3-targeting shRNA #3	GCTCCTGCTTACCTTGCTCTA	Figures 2, 3, S2, S5–S9	MISSION-shRNA library; TRCN0000003495
MSTN-targeting shRNA #1	CCCACAAAGATGTCTCCAATT	Figure S3	MISSION-shRNA library; TRCN0000059138
MSTN-targeting shRNA #2	CCTGAATCCAACTTAGGCATT	Figures 2, 3, S3, S5–S9	MISSION-shRNA library; TRCN0000059140
DMD-targeting shRNA #2	CCAGCATTACTGCCAAAGTTT	Figure S4	MISSION-shRNA library; TRCN0000053245
DMD-targeting shRNA #3	CCAGTCTTTAGCTGACCTGAA	Figures 2, 3, S4, S5–S9	MISSION-shRNA library; TRCN0000053246
DMD-targeting shRNA #4	CCCTAGTTCAAGAGGAAGAAA	Figure 4	MISSION-shRNA library; TRCN0000053243
Micro-dystrophin	Micro-dystrophin	Figure 4	Chamberlain lab; Addgene plasmid #26810
GFP	GFP	Figure 4

ISSN: 2044-5040