Figure 2
From: Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes
Expression profiles of genes upregulated during skeletal muscle differentiation in C 2 C 12 cells. (A) Expression profiles of individual gene expression. Shown are graphs representing the change in expression of each gene examined in this study observed at each day of differentiation with respect to the expression level detected in proliferating C2C12 myoblast cells. (B) The relative transcription levels of each of the genes in this study under conditions of proliferation or differentiation are shown. Expression of each gene was determined by quantitative real-time polymerase chain reaction (qRT-PCR) assay and normalized to HPRT levels. Numbers indicate the fold change between undifferentiated samples (MB, myoblasts) and samples differentiated for six days (MT, myotubes). (A and B) Calculations of the relative fold changes in gene expression and mRNA expression are described in Methods. qRT-PCR assays were performed in triplicate on cDNA samples derived from independent RNA isolations. All data are normalized to the expression level of HPRT. Error bars represent standard deviations of the mean.