Skip to main content
Figure 3 | Skeletal Muscle

Figure 3

From: Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes

Figure 3

Chromatin immunoprecipitation (ChIP) analysis of the muscle creatine kinase ( Ckm ) promoter. Cross-linked extracts from proliferating myoblasts (UD, undifferentiated cells) and myofibers in differentiation media for the indicated number of days (D1-D10) were immunoprecipitated with antibodies against myogenin, MyoD, Myf5, HEB, RNA polymerase II (RNAP II), histone H3 acetylated at lysine 9 and/or 18 (H3 Ac9/ 18) or IgG. Immunoprecipitated DNA was purified and amplified with primers specific to the promoter of Ckm. Relative enrichment at the IgH locus was used to normalize the data. The fold enrichment values were calculated as described in Methods.

Back to article page