Co-localization of selected E3 ligases with INI in C2C12 myotubes expressing YFP-Ala16PABPN1. Immunostaining of E3-ligases was visualized with Alexa-594 secondary antibodies. Co-localization with expPABPN1 in myotubes is demonstrated in the merge image. A 2.5X magnification of nuclei containing expPABPN1 aggregates is highlighted in a box. A) Arih1, Asb11 and Ddb1 E3 ligases show consistent co-localization with aggregated YFP-Ala16-PABPN1. B) Trim63 and Fbxo32 E3 ligases show progressively more co-localization with YFP-Ala16-PABPN1 as INI size increases. Scale bar is 10 μm.