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Figure 5 | Skeletal Muscle

Figure 5

From: Caspase-3, myogenic transcription factors and cell cycle inhibitors are regulated by leukemia inhibitory factor to mediate inhibition of myogenic differentiation

Figure 5

Expression of endogenous transcripts for LIF and receptor components and LIF protein in conditioned media during differentiation. Real-time qPCR for transcripts of LIF, LIFR and gp130 showed increased LIF with a peak 24 hours after differentiation, decreased LIFR following differentiation and relatively consistent levels of gp130 throughout differentiation. Western immunoblot showed the presence of 45 kDa glycoslyated LIF in the conditioned media (CM) comprised of growth media at t = 0 but not conditioned media comprised of differentiation media t = 24 or t = 48 hours. The same 45 kDa band was observed in growth media (GM) but not differentiation media (DM) that was not cultured with cells, that is non-conditioned media (NCM).

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