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Figure 6 | Skeletal Muscle

Figure 6

From: Caspase-3, myogenic transcription factors and cell cycle inhibitors are regulated by leukemia inhibitory factor to mediate inhibition of myogenic differentiation

Figure 6

MH35-BD blocks the biological effect of LIF in growth media showing increased differentiation and apoptotic signalling. (A) Creatine kinase activity of myoblasts after 24 hours of differentiation with increasing concentrations of MH35-BD; 100 ng/mL and 1000 ng/mL MH35-BD significantly increased creatine kinase activity. Asterisk (*) indicates P < 0.05 with one-way ANOVA and Dunnet's post-hoc test for all concentrations compared to control (n = 4). (B) The percentage of cells positive for cleaved caspase-3 after 24 hours differentiation was increased with MH35-BD (100 ng/mL) treatment. Asterisk (*) indicates P < 0.05 with Student's t-test compared to control (n = 4). (C) The percentage of cells positive for TUNEL after 24 hours of differentiation was increased with MH35-BD (100 ng/mL) treatment. Asterisk (*) indicates P < 0.05 with Student's t-test compared to control (n = 4).

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Skeletal Muscle

ISSN: 2044-5040

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