Figure 5

Transgenic miR-486 overexpression in mice results in abnormal skeletal muscle regeneration following CTX-induced TA injury. (A) Diagram indicating the construct used to generate MCK-miR-486-transgenic mice. Real-time qPCR of miR-486 expression levels in the TA muscle was analyzed from three separate wild-type (black bars) and miR-486 Tg (red bars) adult mice. Expression levels were normalized to U6 snRNA levels and compared to wild-type (control) levels. (B) Mouse weights of wild-type and miR-486 Tg mice versus age (in months) are shown. Three mouse cohorts of male and female littermates were weighed from 1, 3, 6, 9, 12 and 15 months of age (n = 5 male or female mice per cohort). *P < 0.005 and **P < 0.05. (C) H & E-stained histological sections of wild-type and miR-486 Tg mice on day 0 (uninjured), 7 and 14 post-CTX-induced TA injury. Arrowheads indicate centralized myonuclei. Scale bars = 20 μm. (D) Percentages of total centralized myonuclei during CTX-induced TA injury in wild-type and miR-486 Tg mice. Percentages are based on counts of 200 myofibers in three separate mice per cohort at three time points (0, 7 and 14 days post-CTX injury). *P < 0.005 and **P < 0.05.