Characterization of the PPARδ-cKO model. Blue and green bars represent wild-type (WT) and peroxisome proliferator-activated receptor δ conditional knockout (Pparδ-cKO) (MUT) tissues, respectively. (A) Relative expression levels of Pparδ in tibialis anterior (TA) muscle, brown fat and white fat tissues (N = 8 pairs of wild-type and mutant mice) for each tissue type. (B) Quantitative RT-PCR showing the relative expression levels of Pparδ in whole-muscle tissue, proliferating myoblasts, white fat and brown fat in wild-type mice (N = 8 for each tissue type and N = 2 for myoblasts). (C) and (D) Expression levels of Pparα in TA muscle (C) and Pparγ in TA, brown fat and white fat tissues (N = 8 for each tissue type) (D). (E) and (F) Expression levels of carnitine palmitoyltransferase 1β (mCPT1β) and Forkhead box class O transcription factor 1 (FoxO1) genes in TA muscles (N = 3).