Figure 1

Dbn1 is upregulated during myoblast differentiation in an SB203580-sensitive manner. (A) Primary mouse myoblasts proliferating in growth medium (G) or transferred to differentiation medium for the indicated times in the presence (+) or absence (-) of 5 μM SB203580 were analyzed by quantitative RT-PCR for expression Dbn1, Myog (encoding myogenin) and Myh3 (encoding myosin heavy chain 3; MHC3). (B) Cultures parallel to those in part (A) were examined by Western blot analysis for drebrin, myogenin and MHC production. Tubulin was used as a loading control. (C) C2C12 myoblasts were analyzed similarly to the primary myoblasts shown in part (B).