Figure 2
From: Regulation of myotube formation by the actin-binding factor drebrin
RNA interference (RNAi)-mediated depletion of drebrin reduces C2C12 cell differentiation. (A) C2C12 cells were transfected with either pSUPER (control) or pSUPER containing RNAi sequences targeted against Dbn1 (siDbn1-1, siDbn1-3). After 3 days in differentiation medium (DM), cultures were fixed and double-stained for myosin heavy chain (red) and 4',6-diamidino-2-phenylindole (blue). Bar: 0.1 mm. (B) Quantification of myotube formation. Values shown are means ± SD of triplicate determinations. *P < 0.05. (C) Western blot analysis of C2C12 cells expressing the indicated control or Dbn1 siRNA vectors cultured in growth medium (G) or DM for the indicated times.