Figure 4

Cell proliferation and apoptosis in primary myoblasts depleted of drebrin. (A) Primary myoblatsts were transfected with control or Dbn1-specific siRNA. Cells were cultured for 36 hours in growth medium (GM), then exposed to bromodeoxyuridine (BrdU) for 2 hours and finally fixed and stained with antibody against BrdU. Values shown are means ± SD of triplicate determinations. (B) Primary myoblasts were transfected with control (Con) or Dbn1-specific siRNA. Cells were cultured in GM for 36 hours or in differentiation medium for 48 hours. Values shown are means ± SD of triplicate determinations. *P < 0.01 by Student's t-test.