Figure 2
From: Skeletal muscle proteomics: current approaches, technical challenges and emerging techniques
Proteomic profiling of skeletal muscle. The flowchart outlines the various preparative and analytical steps involved in the routine mass spectrometry-based proteomic investigation of contractile tissues. Protein separation is usually carried out by one-dimensional polyacrylamide gel electrophoresis (1-D PAGE), 2-D gel electrophoresis with isoelectric focusing in the first dimension and PAGE in the second slab gel dimension (2-D IEF-PAGE) and/or liquid chromatography.