Figure 5
From: Skeletal muscle proteomics: current approaches, technical challenges and emerging techniques
Detergent phase extraction method for muscle proteomics. The flowchart outlines the application of detergent phase extraction for the mass spectrometric identification of membrane-associated proteins from skeletal muscle tissues. The separation of protein species into an aqueous (AQ) fraction versus a detergent (DT) fraction can be conveniently verified by immunoblotting with antibodies to soluble versus integral markers.