Figure 2

Cell-cell association duration and distribution. Of 244 tracked cells, 176 showed interactions with either a mitotic sister or an unrelated cell during the observation timeframe. (a) Average time (out of 24 hours) of associations between either 'sisters' (products of the same cell division) or 'strangers' (unrelated cells). Note that for sister cells, the associations immediately after cell division and total time including later reassociations are shown separately, whereas for unrelated cells, the total interaction time with any unrelated cell and the average association time with individual unrelated cells are shown separately. (b) Of the 176 cells, 66 interacted with unrelated satellite cells; most showed interactions with either one or two unrelated cells but some contacted up to four unrelated cells. (c) Average time (out of 24 hours) of association between sister cells that were the product of a horizontal division versus sisters from a vertical division, broken down by cell division number. Daughters of a vertical division were significantly more likely to remain associated for an extended period of time after cell division [P < 10^-8, except for third-division cells, for which significance was poor because of the small sample size (only two cells dividing for the third time underwent vertical division)]. Daughters of the first observed division had an enhanced tendency to remain associated. Association time immediately after division and total time for all association between sister cells are shown separately.