Figure 2

A. Muscle age profiling with HIV. Heatmap displaying the ten gene profile shown in Figure 1, with the HIV group added using GSE1428 (Figure 2A) and GSE362 (similar results, data not shown). The CAGED software was used to cluster samples (shown as rows of the heat map) based on the ten gene muscle aging profile (columns of the heat map). The analysis shows that the young men form a cluster, while HIV samples (designated by arrows and in bold, 36 to 51 years old) cluster with old subjects using the ten gene muscle aging profile. The CAGED cluster analysis uses a model-based procedure that assigns samples to the same cluster if their merging increases the posterior probability of the model. Numbers attached to the branches of the dendrogram represent the posterior odds of the model that merges the branches versus the model that does not. B. CAGED analysis of the expression of the ten gene muscle aging profile is an HIV negative dataset with young (20 to 25 years old), intermediate (40 to 45 years old designated by arrows), and old (70 to 75 years old) subjects (this entire dataset used the microarray NimbleGen platform). The gene expression of the profile is displayed in a supervised manner where intensity reflects relative expression (green = higher than average, red = lower than average). CAGED, Cluster Analysis of Gene Expression Dynamics