Figure 3

Expression of p21/Cip1, p16INK4a and TGFβ1 in the HIV transgenic rodent. A. Realtime RNA PCR validation was observed for p21/Cip1, Fez2, H3, MFL1, MT1F and MYH8 in muscle from HIV transgenic gastrocnemius (n = 4) muscle or wild type gastrocnemius muscle (n = 3) and shows an increase in genes in HIV Tg compared to age matched wild type controls. All bar plots show mean fold change with error bars indicating the standard deviation. The difference between wild type and HIV Tg is significant to P ≤ 0.05. B. RT-PCR shows an increase in the cell cycle arrest gene, p16INK4a, in HIV Tg rat (n =4) compared to wild type (n = 3). The difference is significant to P < 0.05. The bar plot indicates mean fold change with the error bars indicative of the standard deviation. C. TGFβ1 protein levels based on ELISA of muscle homogenates from HIV transgenic gastrocnemius muscle (n = 3) or wild type gastrocnemius muscle (n = 3) show increased levels in the HIV Tg rodent at a significance of P < 0.05. Bar plots indicate mean protein levels with error bars indicative of the standard deviation. TGFβ, transforming growth factor β