Figure 5
Colony-forming cell (CFC) analysis of different muscle groups. (A) Cloning efficiency for all five muscle groups across three replicates (independent mice). Cloning efficiency represents the percentage of wells in a 96-well plate that show satellite cell colony formation. Each data point represents one plate. Blue bars represent the mean. (B) The total number of nuclei per colony for each of five muscle groups. Blue bars represent mean and 95% confidence intervals. (C) The coefficient of differentiation, Df for all five muscle groups. Blue bars represent mean and 95% confidence intervals. (D) The fusion index, Ui for all five muscle groups. Blue bars represent mean and 95% confidence intervals. (E) Example colonies. Left: typical diaphragm colony. Right: typical dorsi colony showing high differentiation percentage. Blue bars represent mean and 95% confidence intervals. Diaph = diaphragm; TA = tibialis anteriors.