Neuromuscular junctions (NMJs) in Eps15 homology domain-containing (EHD)1−/−mice show no morphological defects in synaptic endplate structure. (A) The tibialis anterior (TA) skeletal muscle fibers from three EHD1−/− and three wild-type (WT) mice were labeled with α-bungarotoxin (BTX) Alexa Fluor 555 or 488 conjugate for morphological mapping of endplates using confocal microscopy. NMJs were seen in both WT (n = 260) and in EHD1−/− (n = 275) fibers. Blue, DAPI; green, BTX Alexa Fluor 488 conjugate; red, anti-rabbit Alexa Fluor 555 conjugate. Scale bars: 20 μm. A larger reference bar was added above the scale bars.