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Figure 1 | Skeletal Muscle

Figure 1

From: TNF-α- and tumor-induced skeletal muscle atrophy involves sphingolipid metabolism

Figure 1

Effects of tumor necrosis factor (TNF)-α and exogenous ceramide on L6 myotube characteristics. (A) Differentiated myotubes treated for 3 days with 15 ng/ml TNF-α or 5 μmol/l C6 ceramide were immunostained for sarcomeric myosin heavy chain (MHC), and their surfaces were evaluated by using Image J software. The graph shows the myotube area (mean ± SE) expressed as a percentage of the field surface, as determined in ten fields, and is representative of at least three experiments. Different from control: **P = 0.01; ***P = 0.001. (B) The MHC content of myotubes treated for 3 consecutive days with TNF-α or C6 ceramide at the indicated concentrations was evaluated by ELISA. The mean ± SE of three measurements expressed as a percentage of control values is shown. The mean control value was 75 ± 11 μg of MHC per 120,000 cells. * Different from control: P < 0.05; P ≤ 0.001. (C) The creatine kinase activity of myotubes treated for 3 days with 15 ng/ml TNF-α or 5 μmol/l C6 ceramide was assayed in triplicate (mean ± SE).*Different from control: P < 0.05.

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